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1.
Sci Rep ; 14(1): 10674, 2024 May 09.
Article in English | MEDLINE | ID: mdl-38724584

ABSTRACT

Accurate development of satellite maneuvers necessitates a broad orbital dynamical system and efficient nonlinear control techniques. For achieving the intended formation, a framework of a discrete fractional difference satellite model is constructed by the use of commensurate and non-commensurate orders for the control and synchronization of fractional-order chaotic satellite system. The efficacy of the suggested framework is evaluated employing a numerical simulation of the concerning dynamic systems of motion while taking into account multiple considerations such as Lyapunov exponent research, phase images and bifurcation schematics. With the aid of discrete nabla operators, we monitor the qualitative behavioural patterns of satellite systems in order to provide justification for the structure's chaos. We acquire the fixed points of the proposed trajectory. At each fixed point, we calculate the eigenvalue of the satellite system's Jacobian matrix and check for zones of instability. The outcomes exhibit a wide range of multifaceted behaviours resulting from the interaction with various fractional-orders in the offered system. Additionally, the sample entropy evaluation is employed in the research to determine complexities and endorse the existence of chaos. To maintain stability and synchronize the system, nonlinear controllers are additionally provided. The study highlights the technique's vulnerability to fractional-order factors, resulting in exclusive, changing trends and equilibrium frameworks. Because of its diverse and convoluted behaviour, the satellite chaotic model is an intriguing and crucial subject for research.

2.
Sci Rep ; 14(1): 8827, 2024 04 17.
Article in English | MEDLINE | ID: mdl-38632309

ABSTRACT

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel virus known as coronavirus 2 (SARS-CoV-2) that affects the pulmonary structure and results in the coronavirus illness 2019 (COVID-19). Tuberculosis (TB) and COVID-19 codynamics have been documented in numerous nations. Understanding the complexities of codynamics is now critically necessary as a consequence. The aim of this research is to construct a co-infection model of TB and COVID-19 in the context of fractional calculus operators, white noise and probability density functions, employing a rigorous biological investigation. By exhibiting that the system possesses non-negative and bounded global outcomes, it is shown that the approach is both mathematically and biologically practicable. The required conditions are derived, guaranteeing the eradication of the infection. Sensitivity analysis and bifurcation of the submodel are also investigated with system parameters. Furthermore, existence and uniqueness results are established, and the configuration is tested for the existence of an ergodic stationary distribution. For discovering the system's long-term behavior, a deterministic-probabilistic technique for modeling is designed and operated in MATLAB. By employing an extensive review, we hope that the previously mentioned approach improves and leads to mitigating the two diseases and their co-infections by examining a variety of behavioral trends, such as transitions to unpredictable procedures. In addition, the piecewise differential strategies are being outlined as having promising potential for scholars in a range of contexts because they empower them to include particular characteristics across multiple time frame phases. Such formulas can be strengthened via classical technique, power-law, exponential decay, generalized Mittag-Leffler kernels, probability density functions and random procedures. Furthermore, we get an accurate description of the probability density function encircling a quasi-equilibrium point if the effect of TB and COVID-19 minimizes the propagation of the codynamics. Consequently, scholars can obtain better outcomes when analyzing facts using random perturbations by implementing these strategies for challenging issues. Random perturbations in TB and COVID-19 co-infection are crucial in controlling the spread of an epidemic whenever the suggested circulation is steady and the amount of infection eliminated is closely correlated with the random perturbation level.


Subject(s)
COVID-19 , Coinfection , Tuberculosis , Humans , SARS-CoV-2 , Coinfection/epidemiology , Tuberculosis/epidemiology , Mathematics
3.
Sci Rep ; 13(1): 22447, 2023 12 17.
Article in English | MEDLINE | ID: mdl-38105245

ABSTRACT

Complex networks have been programmed to mimic the input and output functions in multiple biophysical algorithms of cortical neurons at spiking resolution. Prior research has demonstrated that the ineffectual features of membranes can be taken into account by discrete fractional commensurate, non-commensurate and variable-order patterns, which may generate multiple kinds of memory-dependent behaviour. Firing structures involving regular resonator chattering, fast, chaotic spiking and chaotic bursts play important roles in cortical nerve cell insights and execution. Yet, it is unclear how extensively the behaviour of discrete fractional-order excited mechanisms can modify firing cell attributes. It is illustrated that the discrete fractional behaviour of the Izhikevich neuron framework can generate an assortment of resonances for cortical activity via the aforesaid scheme. We analyze the bifurcation using fragmenting periodic solutions to demonstrate the evolution of periods in the framework's behaviour. We investigate various bursting trends both conceptually and computationally with the fractional difference equation. Additionally, the consequences of an excitable and inhibited Izhikevich neuron network (INN) utilizing a regulated factor set exhibit distinctive dynamic actions depending on fractional exponents regulating over extended exchanges. Ultimately, dynamic controllers for stabilizing and synchronizing the suggested framework are shown. This special spiking activity and other properties of the fractional-order model are caused by the memory trace that emerges from the fractional-order dynamics and integrates all the past activities of the neuron. Our results suggest that the complex dynamics of spiking and bursting can be the result of the long-term dependence and interaction of intracellular and extracellular ionic currents.


Subject(s)
Models, Neurological , Neurons , Action Potentials/physiology , Neurons/physiology , Biophysics , Neural Networks, Computer
4.
J Pak Med Assoc ; 73(11): 2273-2276, 2023 Nov.
Article in English | MEDLINE | ID: mdl-38013546

ABSTRACT

Hunter syndrome (mucopolysaccharidosis type II) has the highest reported prevalence of difficult tracheal intubation among the seven known types of mucopolysaccharidoses. Despite improved difficult airway guidelines and equipment, conventional approaches may fail in some cases. A 10-year-old child with Hunter syndrome, was scheduled for multiple dental extractions. On the first visit, failed intubation was declared as per Difficult Airway Society guidelines in the surgical day-care suite of our institute and the procedure was postponed. The case was then planned to be handled in the main operating room with additional preparation and input from the paediatric otolaryngologist for possible tracheostomy, paediatric intensive care for postoperative need for ventilation, and difficult airway resource faculty for an unconventional approach-videolaryngoscope combined with fibreoptic bronchoscope-which resulted in safe administration of anaesthesia. This case illustrates the importance of meticulous planning in the management of previously failed airway.


Subject(s)
Anesthesia , Laryngoscopes , Mucopolysaccharidosis II , Humans , Child , Bronchoscopy , Mucopolysaccharidosis II/complications , Mucopolysaccharidosis II/therapy , Intubation, Intratracheal , Fiber Optic Technology
5.
Sci Rep ; 13(1): 18180, 2023 10 24.
Article in English | MEDLINE | ID: mdl-37875469

ABSTRACT

The multiple activities of neurons frequently generate several spiking-bursting variations observed within the neurological mechanism. We show that a discrete fractional-order activated nerve cell framework incorporating a Caputo-type fractional difference operator can be used to investigate the impacts of complex interactions on the surge-empowering capabilities noticed within our findings. The relevance of this expansion is based on the model's structure as well as the commensurate and incommensurate fractional-orders, which take kernel and inherited characteristics into account. We begin by providing data regarding the fluctuations in electronic operations using the fractional exponent. We investigate two-dimensional Morris-Lecar neuronal cell frameworks via spiked and saturated attributes, as well as mixed-mode oscillations and mixed-mode bursting oscillations of a decoupled fractional-order neuronal cell. The investigation proceeds by using a three-dimensional slow-fast Morris-Lecar simulation within the fractional context. The proposed method determines a method for describing multiple parallels within fractional and integer-order behaviour. We examine distinctive attribute environments where inactive status develops in detached neural networks using stability and bifurcation assessment. We demonstrate features that are in accordance with the analysis's findings. The Erdös-Rényi connection of asynchronization transformed neural networks (periodic and actionable) is subsequently assembled and paired via membranes that are under pressure. It is capable of generating multifaceted launching processes in which dormant neural networks begin to come under scrutiny. Additionally, we demonstrated that boosting connections can cause classification synchronization, allowing network devices to activate in conjunction in the future. We construct a reduced-order simulation constructed around clustering synchronisation that may represent the operations that comprise the whole system. Our findings indicate the influence of fractional-order is dependent on connections between neurons and the system's stored evidence. Moreover, the processes capture the consequences of fractional derivatives on surge regularity modification and enhance delays that happen across numerous time frames in neural processing.


Subject(s)
Algorithms , Neural Networks, Computer , Computer Simulation , Biophysics , Neurons/physiology
7.
Sci Rep ; 13(1): 14824, 2023 09 08.
Article in English | MEDLINE | ID: mdl-37684316

ABSTRACT

To boost the handful of nutrient-dense individuals in the societal structure, adequate health care documentation and comprehension are permitted. This will strengthen and optimize the well-being of the community, particularly the girls and women of the community that are welcoming the new generation. In this article, we extensively explored a deterministic-stochastic malnutrition model involving nonlinear perturbation via piecewise fractional operators techniques. This novel concept leads us to analyze and predict the process from the beginning to the end of the well-being growth, as it offers the possibility to observe many behaviors from cross over to stochastic processes. Moreover, the piecewise differential operators, which can be constructed with operators such as classical, Caputo, Caputo-Fabrizio, Atangana-Baleanu and stochastic derivative. The threshold parameter is developed and the role of malnutrition in society is examined. Through a rigorous analysis, we first demonstrated that the stochastic model's solution is positive and global. Then, using appropriate stochastic Lyapunov candidates, we examined whether the stochastic system acknowledges a unique ergodic stationary distribution. The objective of this investigation is to design a nutritional deficiency in pregnant women using a piecewise fractional differential equation scheme. We examined multiple options and outlined numerical methods of coping with problems. To exemplify the effectiveness of the suggested concept, graphical conclusions, including chaotic and random perturbation patterns, are supplied. Consequently, fractional calculus' innovative aspects provide more powerful and flexible layouts, enabling us to more effectively adapt to the system dynamics tendencies of real-world representations. This has opened new doors to readers in different disciplines and enabled them to capture different behaviors at different time intervals.


Subject(s)
Adaptation, Psychological , Malnutrition , Pregnancy , Humans , Female , Documentation , Health Facilities , Nutrients
8.
Sci Rep ; 13(1): 15320, 2023 09 15.
Article in English | MEDLINE | ID: mdl-37714907

ABSTRACT

Lassa fever is a hemorrhagic virus infection that is usually spread by rodents. It is a fatal infection that is prevalent in certain West African countries. We created an analytical deterministic-stochastic framework for the epidemics of Lassa fever employing a collection of ordinary differential equations with nonlinear solutions to identify the influence of propagation processes on infected development in individuals and rodents, which include channels that are commonly overlooked, such as ecological emergent and aerosol pathways. The findings shed light on the role of both immediate and subsequent infectiousness via the power law, exponential decay and generalized Mittag-Leffler kernels. The scenario involves the presence of a steady state and an endemic equilibrium regardless of the fundamental reproduction number, [Formula: see text], making Lassa fever influence challenging and dependent on the severity of the initial sub-populations. Meanwhile, we demonstrate that the stochastic structure has an exclusive global positive solution via a positive starting point. The stochastic Lyapunov candidate approach is subsequently employed to determine sufficient requirements for the existence and uniqueness of an ergodic stationary distribution of non-negative stochastic simulation approaches. We acquire the particular configuration of the random perturbation associated with the model's equilibrium [Formula: see text] according to identical environments as the presence of a stationary distribution. Ultimately, modeling techniques are used to verify the mathematical conclusions. Our fractional and stochastic findings exhibit that when all modes of transmission are included, the impact of Lassa fever disease increases. The majority of single dissemination pathways are less detrimental with fractional findings; however, when combined with additional spread pathways, they boost the Lassa fever stress.


Subject(s)
Epidemics , Lassa Fever , Humans , Africa, Western , Computer Simulation
10.
Results Phys ; 49: 106467, 2023 Jun.
Article in English | MEDLINE | ID: mdl-37153140

ABSTRACT

The SARSCoV-2 virus, also known as the coronavirus-2, is the consequence of COVID-19, a severe acute respiratory syndrome. Droplets from an infectious individual are how the pathogen is transmitted from one individual to another and occasionally, these particles can contain toxic textures that could also serve as an entry point for the pathogen. We formed a discrete fractional-order COVID-19 framework for this investigation using information and inferences from Thailand. To combat the illnesses, the region has implemented mandatory vaccination, interpersonal stratification and mask distribution programs. As a result, we divided the vulnerable people into two groups: those who support the initiatives and those who do not take the influence regulations seriously. We analyze endemic problems and common data while demonstrating the threshold evolution defined by the fundamental reproductive quantity R 0 . Employing the mean general interval, we have evaluated the configuration value systems in our framework. Such a framework has been shown to be adaptable to changing pathogen populations over time. The Picard Lindelöf technique is applied to determine the existence-uniqueness of the solution for the proposed scheme. In light of the relationship between the R 0 and the consistency of the fixed points in this framework, several theoretical conclusions are made. Numerous numerical simulations are conducted to validate the outcome.

11.
World J Radiol ; 15(4): 127-135, 2023 Apr 28.
Article in English | MEDLINE | ID: mdl-37181823

ABSTRACT

BACKGROUND: Prominent leptomeningeal contrast enhancement (LMCE) in the brain is observed in some pediatric patients during sedation for imaging. However, based on clinical history and cerebrospinal fluid analysis, the patients are not acutely ill and do not exhibit meningeal signs. Our study determined whether sevoflurane inhalation in pediatric patients led to this pattern of 'pseudo' LMCE (pLMCE) on 3 Tesla magnetic resonance imaging (MRI). AIM: To highlight the significance of pLMCE in pediatric patients undergoing enhanced brain MRI under sedation to avoid misinterpretation in reports. METHODS: A retrospective cross-sectional evaluation of pediatric patients between 0-8 years of age was conducted. The patients underwent enhanced brain MRI under inhaled sevoflurane. The LMCE grade was determined by two radiologists, and interobserver variability of the grade was calculated using Cohen's kappa. The LMCE grade was correlated with duration of sedation, age and weight using the Spearman rho rank correlation. RESULTS: A total of 63 patients were included. Fourteen (22.2%) cases showed mild LMCE, 48 (76.1%) cases showed moderate LMCE, and 1 case (1.6%) showed severe LMCE. We found substantial agreement between the two radiologists in detection of pLMCE on post-contrast T1 imaging (kappa value = 0.61; P < 0.001). Additionally, we found statistically significant inverse and moderate correlations between patient weight and age. There was no correlation between duration of sedation and pLMCE. CONCLUSION: pLMCE is relatively common on post-contrast spin echo T1-weighted MRI of pediatric patients sedated by sevoflurane due to their fragile and immature vasculature. It should not be misinterpreted for meningeal pathology. Knowing pertinent clinical history of the child is an essential prerequisite to avoid radiological overcalling and the subsequent burden of additional investigations.

12.
Turk J Anaesthesiol Reanim ; 51(1): 30-36, 2023 Feb.
Article in English | MEDLINE | ID: mdl-36847316

ABSTRACT

OBJECTIVE: Placenta previa is associated with maternal and neonatal morbidity and mortality. This study aims to add to the limited literature from the developing world on the association of different anaesthetic techniques with blood loss, the need for blood transfusion, and maternal/ neonatal outcomes among women undergoing caesarean section with placenta previa. METHODS: This retrospective study was conducted at Aga University Hospital, Karachi, Pakistan. The patient population included parturients undergoing caesarean section for placenta previa from January 1, 2006, through December 31, 2019. RESULTS: Out of 276 consecutive cases of placenta previa progressing to caesarean section during the study period, 36.24% were performed under regional anaesthesia and 63.76% under general anaesthesia. When compared to general anaesthesia, significantly less regional anaesthesia was used for emergency caesarean section (26% vs. 38.6%, P = .033) and for grade IV placenta previa (50% vs. 68.8%, P = .013). Blood loss was found to be significantly low with regional anaesthesia (P = .005) and posterior placenta (P = .042), while it was found to be high in grade IV placenta previa (P = .024). The odds of requiring blood transfusion were low in regional anaesthesia (odds ratio = 0.122; 95% CI = 0.041-0.36, P = .0005) and posterior placenta (odds ratio = 0.402; 95% CI = 0.201-0.804, P = .010), while they were high in grade IV placenta previa (odds ratio: 4.13; 95% CI = 0.90-19.80, P = .0681). The rate of neonatal deaths and intensive care admission was significantly lower in regional anaesthesia than in general anaesthesia (7% vs. 3% and 9% vs. 3%). The maternal mortality was zero; however, intensive care admission was less in regional anaesthesia compared to general anaesthesia (<1% vs. 4%). CONCLUSION: Our data demonstrated less blood loss, need for blood transfusion, and better maternal and neonatal outcomes with regional anaesthesia for caesarean section in women with placenta previa.

13.
Plant Physiol Biochem ; 196: 668-682, 2023 Mar.
Article in English | MEDLINE | ID: mdl-36801772

ABSTRACT

Biostimulants such as ascorbic acid (AA) and fulvic acid (FA)can enhance the efficiency of root-nodulating bacteria. This study investigates optimum concentration of these two biostimulants to maximize the Rhizobium activity and increase root size, nodulation capability, NPK uptake, yield and quality. Interaction with nitrogenase enzyme through molecular docking was also studied by using both AA and FA as ligands to better understand their inhibitory role in excess amounts. The findings of the study suggest: the combined application of both FA and AA at 200 ppm concentrations proved to be more effective than the individual application. Excellent vegetative growth was noticed which translated into an increased reproductive growth i.e statistically significant increase in number of pods per plant, fresh and dry weight of pods per plant, number of seeds per pod, total chlorophyll, carotenoids and chemical constituents of pea seeds i.e. N (16.17%), P (40.47%), K (39.96%) and protein (16.25%). These findings were substantiated by molecular docking of nitrogenase enzyme with ascorbic acid and fulvic acid. The XP docking score of ascorbic acid (-7.07 kcal mol-1) and fulvic acid (-6.908 kcal mol-1) exhibited that the optimum doses (200 ppm) should be used as higher dose or their excess amount can hinder the Rhizobium activity of nitrogen fixation by interacting with the nitrogenase enzyme.


Subject(s)
Meteoroids , Rhizobium , Pisum sativum/metabolism , Ascorbic Acid/pharmacology , Molecular Docking Simulation , Nitrogenase/metabolism
14.
Math Biosci Eng ; 20(1): 402-436, 2023 01.
Article in English | MEDLINE | ID: mdl-36650772

ABSTRACT

Monkeypox (MPX) is a zoonotic illness that is analogous to smallpox. Monkeypox infections have moved across the forests of Central Africa, where they were first discovered, to other parts of the world. It is transmitted by the monkeypox virus, which is a member of the Poxviridae species and belongs to the Orthopoxvirus genus. In this article, the monkeypox virus is investigated using a deterministic mathematical framework within the Atangana-Baleanu fractional derivative that depends on the generalized Mittag-Leffler (GML) kernel. The system's equilibrium conditions are investigated and examined for robustness. The global stability of the endemic equilibrium is addressed using Jacobian matrix techniques and the Routh-Hurwitz threshold. Furthermore, we also identify a criterion wherein the system's disease-free equilibrium is globally asymptotically stable. Also, we employ a new approach by combining the two-step Lagrange polynomial and the fundamental concept of fractional calculus. The numerical simulations for multiple fractional orders reveal that as the fractional order reduces from 1, the virus's transmission declines. The analysis results show that the proposed strategy is successful at reducing the number of occurrences in multiple groups. It is evident that the findings suggest that isolating affected people from the general community can assist in limiting the transmission of pathogens.


Subject(s)
Monkeypox virus , Mpox (monkeypox) , Humans , Mpox (monkeypox)/epidemiology , Social Group
15.
J Coll Physicians Surg Pak ; 32(8): S195-S196, 2022 Aug.
Article in English | MEDLINE | ID: mdl-36210693

ABSTRACT

Thoraco-abdominal aortic aneurysm (TAAA) repair is a complicated and challenging surgery with high chances of unpredictable post-operative outcomes. This is a case of a 56-year man electively admitted for De Bakey type III TAAA repair. The case was done with the one-lung ventilation (OLV) technique by using a bronchial blocker. The other unique thing which was done in this case was the use of a lumbar drain for spinal cord protection. The patient had a prolonged hospital stay but ultimately recovered and was discharged home after two and a half months. Key Words: Lumber drain, Spinal cord injury, Aortic aneurysm surgery.


Subject(s)
Aortic Aneurysm, Abdominal , Aortic Aneurysm, Thoracic , Blood Vessel Prosthesis Implantation , Aortic Aneurysm, Abdominal/surgery , Aortic Aneurysm, Thoracic/surgery , Drainage , Humans , Male , Spinal Cord , Treatment Outcome , Vascular Surgical Procedures
16.
Math Biosci Eng ; 19(11): 11563-11594, 2022 08 12.
Article in English | MEDLINE | ID: mdl-36124604

ABSTRACT

In this paper, the global complexities of a stochastic virus transmission framework featuring adaptive response and Holling type II estimation are examined via the non-local fractal-fractional derivative operator in the Atangana-Baleanu perspective. Furthermore, we determine the existence-uniqueness of positivity of the appropriate solutions. Ergodicity and stationary distribution of non-negative solutions are carried out. Besides that, the infection progresses in the sense of randomization as a consequence of the response fluctuating within the predictive case's equilibria. Additionally, the extinction criteria have been established. To understand the reliability of the findings, simulation studies utilizing the fractal-fractional dynamics of the synthesized trajectory under the Atangana-Baleanu-Caputo derivative incorporating fractional-order α and fractal-dimension ℘ have also been addressed. The strength of white noise is significant in the treatment of viral pathogens. The persistence of a stationary distribution can be maintained by white noise of sufficient concentration, whereas the eradication of the infection is aided by white noise of high concentration.


Subject(s)
Fractals , Virus Diseases , Computer Simulation , Humans , Models, Biological , Reproducibility of Results
17.
Cureus ; 14(7): e26548, 2022 Jul.
Article in English | MEDLINE | ID: mdl-35936146

ABSTRACT

Background and objective The parents of pediatric patients admitted for elective surgery exhibit significant levels of anxiety. The reduction in parental anxiety is directly proportional to the information and counseling provided to the parents preoperatively. The parenting style in South Asian culture is different from that of western cultures and may influence the response to these interventions. In this study, we aimed to compare the mean anxiety levels between parents of children (aged three to eight years) undergoing outpatient infraumbilical surgery equipped with standardized preoperative parental education and those without. Methods This was a randomized, controlled, and blinded trial. Parents of 72 pediatric patients (aged three to eight years) undergoing elective infraumbilical daycare surgery were enrolled and were divided into two groups: an intervention (Group I) and a non-intervention (Group NI) group. Both groups received routine verbal counseling at the preoperative clinic, but a standardized brochure was provided only to Group I. Parental anxiety was measured by using the Visual Analog Scale (VAS) at three different time points: in the outpatient surgery suite on the day of surgery, in the preoperative area 10 minutes before shifting the child to the operating room, and finally in the recovery room. Results The baseline mean VAS score was significantly higher in Group I compared to Group NI (p=0.017). After the intervention, the mean pain score significantly decreased from baseline in Group I as compared to Group NI (mean ±SD: 4.08 ±1.6 vs. 6.08 ±1.66; p=0.0005). Conclusion The information provided through standardized written material to the parents on the day of surgery before anesthesia helped to significantly attenuate preoperative parental anxiety.

18.
Sci Rep ; 12(1): 12404, 2022 07 20.
Article in English | MEDLINE | ID: mdl-35858974

ABSTRACT

Identifying medication errors is one method of improving patient safety. Peri operative anesthetic management of patient includes polypharmacy and the steps followed prior to drug administration. Our objective was to identify, extract and analyze the medication errors (MEs) reported in our critical incident reporting system (CIRS) database over the last 15 years (2004-2018) and to review measures taken for improvement based on the reported errors. CIRS reported from 2004 to 2018 were identified, extracted, and analyzed using descriptive statistics and presented as frequencies and percentages. MEs were identified and entered on a data extraction form which included reporting year, patients age, surgical specialty, American Society of Anesthesiologist (ASA) status, time of incident, phase and type of anesthesia and drug handling, type of error, class of medicine, level of harm, severity of adverse drug event (ADE) and steps taken for improvement. Total MEs reported were 311, medication errors were reported, 163 (52%) errors occurred in ASA II and 90 (29%) ASA III patient, and 133 (43%) during induction. During administration phase 60% MEs occurred and 65% were due to human error. ADEs were found in 86 (28%) reports, 58 of which were significant, 23 serious and five life-threatening errors. The majority of errors involved neuromuscular blockers (32%) and opioids (13%). Sharing of CI and a lesson to be learnt e-mail, colour coded labels, change in medication trolley lay out, decrease in floor stock and high alert labels were the low-cost steps taken to reduce incidents. Medication errors were more frequent during administration. ADEs were occurred in 28% MEs.


Subject(s)
Drug-Related Side Effects and Adverse Reactions , Medication Errors , Drug-Related Side Effects and Adverse Reactions/epidemiology , Humans , Patient Safety , Pharmaceutical Preparations , Retrospective Studies , Risk Management
19.
Front Cell Infect Microbiol ; 12: 764711, 2022.
Article in English | MEDLINE | ID: mdl-35350439

ABSTRACT

SAGA (Spt-Ada-Gcn5-acetyltransferase) is a highly conserved, multiprotein co-activator complex that consists of five distinct modules. It has two enzymatic functions, a histone acetyltransferase (HAT) and a deubiquitinase (DUB) and plays a central role in processes such as transcription initiation, elongation, protein stability, and telomere maintenance. We analyzed conditional and null mutants of the SAGA complex module components in the fungal pathogen Candida albicans; Ngg1, (the HAT module); Ubp8, (the DUB module); Tra1, (the recruitment module), Spt7, (the architecture module) and Spt8, (the TBP interaction unit), and assessed their roles in a variety of cellular processes. We observed that spt7Δ/Δ and spt8Δ/Δ strains have a filamentous phenotype, and both are highly invasive in yeast growing conditions as compared to the wild type, while ngg1Δ/Δ and ubp8Δ/Δ are in yeast-locked state and non-invasive in both YPD media and filamentous induced conditions compared to wild type. RNA-sequencing-based transcriptional profiling of SAGA mutants reveals upregulation of hyphal specific genes in spt7Δ/Δ and spt8Δ/Δ strains and downregulation of ergosterol metabolism pathway. As well, spt7Δ/Δ and spt8Δ/Δ confer susceptibility to antifungal drugs, to acidic and alkaline pH, to high temperature, and to osmotic, oxidative, cell wall, and DNA damage stresses, indicating that these proteins are important for genotoxic and cellular stress responses. Despite having similar morphological phenotypes (constitutively filamentous and invasive) spt7 and spt8 mutants displayed variation in nuclear distribution where spt7Δ/Δ cells were frequently binucleate and spt8Δ/Δ cells were consistently mononucleate. We also observed that spt7Δ/Δ and spt8Δ/Δ mutants were quickly engulfed by macrophages compared to ngg1Δ/Δ and ubp8Δ/Δ strains. All these findings suggest that the SAGA complex modules can have contrasting functions where loss of Spt7 or Spt8 enhances filamentation and invasiveness while loss of Ngg1 or Ubp8 blocks these processes.


Subject(s)
Candida albicans , Transcription Factors , Biofilms , Candida albicans/genetics , Candida albicans/metabolism , Fungal Proteins/genetics , Fungal Proteins/metabolism , Histone Acetyltransferases/genetics , Histone Acetyltransferases/metabolism , Transcription Factors/metabolism
20.
Anat Sci Educ ; 15(2): 376-391, 2022 Mar.
Article in English | MEDLINE | ID: mdl-33914418

ABSTRACT

Recent advance in medical education is in correlation with the advances in information technology and thus computer-based learning is being increasingly employed. The objective of the present study was to design and evaluate an e-learning module in anatomy and assess the perceptions of students and faculty about this e-learning module. The participating students were randomized into three groups by block stratified randomization and Google groups were created for each of the three groups. The e-learning module was implemented in three sessions by rotating the three groups. Validated questionnaires were sent to faculty and participating students via Google forms to obtain feedback. The results of ANOVA showed that there was a significant difference among the groups in terms of marks obtained with conventional (F = 2.403, P = 0.103), online (F = 6.050, P = 0.005), and blended (F = 5.801, P = 0.006). Post hoc comparisons using the Tukey HSD test, about the gain of knowledge, indicated that the results were insignificant when comparing the conventional group with the online group, but were significant when comparing the blended group with the conventional and online group. The qualitative data regarding the perception of students toward e-learning were analyzed using thematic analysis. The introduction of an interactive e-learning module in anatomy was effective and well received by the students and faculty. The study showed that blended learning has a positive impact on the students' learning by improving cognitive gain and receptive perception for e-learning.


Subject(s)
Anatomy , Computer-Assisted Instruction , Anatomy/education , Curriculum , Feasibility Studies , Humans , Learning
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